rabbit anti‐sgo2 antibody  (MyBiosource Biotechnology)

Journal: Frontiers in Oncology

Article Title: The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL)

doi: 10.3389/fonc.2019.00429

Figure Lengend Snippet: RNA-Seq gene expression of meiCT genes (A) STRA8 , (B) STAG3 , (C) SGO2 , (D) SYCP3 , and (E) DMC1 in Sézary Syndrome patients compared to normal/control subjects based on pooled data from Choi et al. and Ungewickell et al. datasets. Data was normalized to a mean TPM value for each cohort. Asterisks indicate statistical significance.

Article Snippet: Immunohistochemistry staining was performed on FFPE tissue sections using the Leica BondTM system and the standard protocol F. Sectioned slides were stained with the following anti-human antibodies: rabbit anti- STRA8 (Novus Biologicals), rabbit anti- STAG3 (Proteintech), rabbit anti- DMC1 (Proteintech), rabbit anti- SGO2 (Novus Biologicals), and rabbit anti- SYCP3 (Abcam) using either heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1 ER1) for 20 min or with an EDTA buffer (pH 9, epitope retrieval solution 2 ER2) for 20 min.

Techniques: RNA Sequencing, Gene Expression, Control

Journal: Frontiers in Oncology

Article Title: The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL)

doi: 10.3389/fonc.2019.00429

Figure Lengend Snippet: RNA-sequencing results for differentially expressed meiCT genes in two pooled independent cohorts of Sézary Syndrome patients ( 12 , 16 ).

Article Snippet: Immunohistochemistry staining was performed on FFPE tissue sections using the Leica BondTM system and the standard protocol F. Sectioned slides were stained with the following anti-human antibodies: rabbit anti- STRA8 (Novus Biologicals), rabbit anti- STAG3 (Proteintech), rabbit anti- DMC1 (Proteintech), rabbit anti- SGO2 (Novus Biologicals), and rabbit anti- SYCP3 (Abcam) using either heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1 ER1) for 20 min or with an EDTA buffer (pH 9, epitope retrieval solution 2 ER2) for 20 min.

Techniques:

Journal: Frontiers in Oncology

Article Title: The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL)

doi: 10.3389/fonc.2019.00429

Figure Lengend Snippet: Results of MeiCT gene expression in lesional skin biopsy samples of patients with CTCL.

Article Snippet: Immunohistochemistry staining was performed on FFPE tissue sections using the Leica BondTM system and the standard protocol F. Sectioned slides were stained with the following anti-human antibodies: rabbit anti- STRA8 (Novus Biologicals), rabbit anti- STAG3 (Proteintech), rabbit anti- DMC1 (Proteintech), rabbit anti- SGO2 (Novus Biologicals), and rabbit anti- SYCP3 (Abcam) using either heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1 ER1) for 20 min or with an EDTA buffer (pH 9, epitope retrieval solution 2 ER2) for 20 min.

Techniques: Gene Expression, Expressing, Staining

Journal: Frontiers in Oncology

Article Title: The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL)

doi: 10.3389/fonc.2019.00429

Figure Lengend Snippet: Immunohistochemistry staining of SGO2 in (A) normal human testis (positive control), (B) normal skin (C) stage IIA MF lesional skin, (D) CD8 + MF lesional skin, (E) Sézary Syndrome ( F) peripheral T-Cell Lymphoma. Scale bars are 50 μm. Nuclear staining in malignant lymphocytes is highlighted (red arrow).

Article Snippet: Immunohistochemistry staining was performed on FFPE tissue sections using the Leica BondTM system and the standard protocol F. Sectioned slides were stained with the following anti-human antibodies: rabbit anti- STRA8 (Novus Biologicals), rabbit anti- STAG3 (Proteintech), rabbit anti- DMC1 (Proteintech), rabbit anti- SGO2 (Novus Biologicals), and rabbit anti- SYCP3 (Abcam) using either heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1 ER1) for 20 min or with an EDTA buffer (pH 9, epitope retrieval solution 2 ER2) for 20 min.

Techniques: Immunohistochemistry, Staining, Positive Control

Journal: Journal of Cancer

Article Title: High SGO2 predicted poor prognosis and high therapeutic value of lung adenocarcinoma and promoted cell proliferation, migration, invasion, and epithelial-to-mesenchymal transformation

doi: 10.7150/jca.86285

Figure Lengend Snippet: SGO2 increases the risk of poor prognosis for LUAD. (A, E, and H) Based on the TCGA database, SGO2 expression, ROC curve, and survival analysis in LUAD. (B, F, and I) Based on the GEO database, the validation dataset GSE30219 was used to verify the SGO2 expression, ROC curve, and survival analysis in LUAD. (C) The expression of SGO2 in LUAD and normal tissue. (D) The protein expression of SGO2 in LUAD and normal tissue. (G and J) Immunohistochemical staining of SGO2 in LUAD and normal tissue.

Article Snippet: The sections were incubated with a 1:1000 rabbit anti-human SGO2 antibody (Cat. # NBP1-83567; Novus, USA) overnight at a 4 °C in wet box.

Techniques: Expressing, Biomarker Discovery, Immunohistochemical staining, Staining

Journal: Journal of Cancer

Article Title: High SGO2 predicted poor prognosis and high therapeutic value of lung adenocarcinoma and promoted cell proliferation, migration, invasion, and epithelial-to-mesenchymal transformation

doi: 10.7150/jca.86285

Figure Lengend Snippet: High SGO2 is associated with a higher TNM stage of LUAD. (A) Based on the TCGA and GSE30219, the expression of SGO2 in T1 vs T2. (B) Based on the TCGA and GSE30219, the expression of SGO2 in N0 vs N1. (C) Based on the TCGA and GSE31210, the expression of SGO2 in stage I vs stage II + III. (D and G) Based on IHC, the protein expression and staining of SGO2 in T1 vs T2. (E and H) Based on IHC, the protein expression and staining of SGO2 in N0 vs N1. (F and I) Based on IHC, the protein expression and staining of SGO2 in stage I vs stage II + III.

Article Snippet: The sections were incubated with a 1:1000 rabbit anti-human SGO2 antibody (Cat. # NBP1-83567; Novus, USA) overnight at a 4 °C in wet box.

Techniques: Expressing, Staining

Journal: Journal of Cancer

Article Title: High SGO2 predicted poor prognosis and high therapeutic value of lung adenocarcinoma and promoted cell proliferation, migration, invasion, and epithelial-to-mesenchymal transformation

doi: 10.7150/jca.86285

Figure Lengend Snippet: SGO2 silencing inhibits the proliferation of lung cancer cells. (A)SGO2 expression after knockdown in A549 and H1299. (B)CCK8 assays were performed to detect A549 and H1299 proliferation. (C)Edu assays were performed to detect A549 and H1299 proliferation.

Article Snippet: The sections were incubated with a 1:1000 rabbit anti-human SGO2 antibody (Cat. # NBP1-83567; Novus, USA) overnight at a 4 °C in wet box.

Techniques: Expressing, Knockdown

Journal: Journal of Cancer

Article Title: High SGO2 predicted poor prognosis and high therapeutic value of lung adenocarcinoma and promoted cell proliferation, migration, invasion, and epithelial-to-mesenchymal transformation

doi: 10.7150/jca.86285

Figure Lengend Snippet: The downregulation of SGO2 affects migration, invasion, and EMT. (A) A Transwell assay was performed to examine the effect of SGO2 knockdown on A549 and H1299 cells. (C) A wound healing assay was performed to examine the effect of SGO2 knockdown on A549 and H1299 cells. (B and D) WB assay the expression levels of E-cadherin, N-cadherin, Vimentin, and Cytokeratin in A549 and H1299 to evaluate the effect on EMT after SGO2 knockdown.

Article Snippet: The sections were incubated with a 1:1000 rabbit anti-human SGO2 antibody (Cat. # NBP1-83567; Novus, USA) overnight at a 4 °C in wet box.

Techniques: Migration, Transwell Assay, Knockdown, Wound Healing Assay, Expressing

Journal: Journal of Cancer

Article Title: High SGO2 predicted poor prognosis and high therapeutic value of lung adenocarcinoma and promoted cell proliferation, migration, invasion, and epithelial-to-mesenchymal transformation

doi: 10.7150/jca.86285

Figure Lengend Snippet: SGO2 expression and tumor immune infiltration. (A) the expression of SGO2 was positively correlated with the infiltration of Memory B cells, Activated CD4+ memory T cells, and CD8+ T cells, while the infiltration of Memory B cells and Tregs decreased with increasing SGO2 expression. (B) Correlation of SGO2 expression with 28 distinct types of tumor-infiltrating immune cells based on ssGSEA.

Article Snippet: The sections were incubated with a 1:1000 rabbit anti-human SGO2 antibody (Cat. # NBP1-83567; Novus, USA) overnight at a 4 °C in wet box.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: High SGO2 predicted poor prognosis and high therapeutic value of lung adenocarcinoma and promoted cell proliferation, migration, invasion, and epithelial-to-mesenchymal transformation

doi: 10.7150/jca.86285

Figure Lengend Snippet: High SGO2 has multiple therapeutic benefits. (A) Comparison of mutational landscapes of SGO between high cluster and low cluster, and Comparison of tumor mutation burden (TMB) between two clusters. (B) Comparison of first-line chemotherapy and targeted therapy drug targets of high and low SGO2 clusters. (C) Comparison of immunomodulatory drug targets of high and low SGO2 clusters.

Article Snippet: The sections were incubated with a 1:1000 rabbit anti-human SGO2 antibody (Cat. # NBP1-83567; Novus, USA) overnight at a 4 °C in wet box.

Techniques: Comparison, Mutagenesis

Journal: Reproductive Medicine and Biology

Article Title: Effects of post‐ovulatory aging on centromeric cohesin protection in murine MII oocytes

doi: 10.1002/rmb2.12433

Figure Lengend Snippet: Comparison of SGO2 expression in mouse oocytes. A, Localization of SGO2 in mouse oocytes. Immunofluorescent staining of SGO2 (green) in the 12‐h aged and fresh oocytes. DNA (blue) was stained with Hoechst 33258. D‐PBS was used as the negative control instead of the primary antibody. Scale bar = 10 μm. B, Comparison of brightness values of SGO2 signal in the 12‐h aged and fresh oocytes. C, Relative expression of SGO2 to α‐tubulin. Bars with different superscripts indicate significant differences ( p < 0.05). D, Western blotting for SGO2 protein in MII oocyte. α‐tubulin was used as a loading control

Article Snippet: The oocyte lysates were diluted with an equal volume of 2× Laemmli sample buffer (Bio‐Rad) containing 5% 2‐mercaptoethanol (Wako) and heated to 100°C for 5 min. Proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) with a stacking gel containing 4% acrylamide (Wako) and a separating gel containing 7.5–10% acrylamide run for 50 min at 200 V. Proteins were then electrophoretically transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare) for 30 min at 15 V. Each membrane was blocked with PVDF blocking reagent (TOYOBO Co., Ltd.) for 1 h at room temperature, followed by incubation overnight at 4 °C with rabbit anti‐p‐REC8 antibody (1:1,000 dilution, LS‐C47114; LifeSpan Biosciences, Inc.), mouse anti‐PP2A‐Aα/β antibody (1:1,000 dilution; Santa Cruz), rabbit anti‐SGO2 antibody (1:1,000 dilution; My BioSource), mouse anti‐BUB1 antibody (1:500 dilution; Santa Cruz), mouse anti‐ARK‐2 antibody (1:500 dilution; Santa Cruz), or goat anti‐MAD2 antibody (1:500 dilution; Santa Cruz) as the primary antibody.

Techniques: Expressing, Staining, Negative Control, Western Blot

Journal: Scientific reports

Article Title: Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas.

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Figure 2. The expression level of SGO2 is related to the survival of patients with high-grade gliomas. The Kaplan–Meier survival curve analyzed from GEO profile (GDS1816/230165_at/SGO2) (a), TCGA (b), and CGGA (c). Data showed that patients with high expression of SGO2 had unfavorable survival outcome. (GDS1816/230165_at/SGO2, n = 77, p = 0.0011 by log-rank test, 95% CI 1.000–1.00, hazard ratio 1.001; TCGA, n = 343, p < 1 × 10–15 by log-rank test, 95% CI 0.87–5.30, hazard ratio 3.90; CGGA, n = 209, p = 1.23 × 10–11 by log- rank test, 95% CI 2.13–4.25, hazard ratio 3.01).

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit antihuman SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Expressing

Journal: Scientific reports

Article Title: Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas.

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Figure 1. The expression level of SGO2 is related to pathological grading of gliomas. The SGO2 mRNA level in different grade of gliomas and non-tumor brain tissue from GEO profile (GDS1962/230165_at/SGO2) (a), TCGA (b), and CCGA (c). SGO2 expression was significantly higher in high-grade gliomas (Grade III and IV) than in low-grade gliomas (Grade II) and non-tumor control. The Y-axis indicates the SGO2 mRNA expression. The p value was adjusted by Bonferroni method in R software (version 3.0.1) between each group.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit antihuman SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Expressing, Control, Software

Journal: Scientific reports

Article Title: Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas.

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Figure 4. Validation of SGO2 protein expression in human gliomas and non-tumor brain tissue. Hematoxylin and eosin staining of non-tumor brain tissue (a), low grade (b) and high grade gliomas (c). The immunohistochemical staining of SGO2 on non-tumor brain tissue (d), low grade (e), and high grade gliomas (f) (scale bar: 50 μm). (g–i) The SGO2 immunostaining scores in normal brain tissue, low-grade glioma and high-grade glioma were statistically analyzed. The adjusted p value was calibrated between each group.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit antihuman SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Biomarker Discovery, Expressing, Staining, Immunohistochemical staining, Immunostaining

Journal: Scientific reports

Article Title: Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas.

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Figure 3. Validation of SGO2 mRNA and protein levels in glioma cell lines and normal brain tissue (a) qRT- PCR was performed to examine SGO2 mRNA expression and the quantitative results are shown in glioma cell lines. The relative expressions were normalized with normal brain. Bars mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.005 showed significant differences. Data are representative of three independent experiments. (b) Protein lysates of glioma cell lines, including U87MG, LN229, GBM8401, and U118MG were applied to SDS-PAGE and Western blot analysis to quantitate SGO2 protein expression (full length blot is presented in Supplementary Fig. 1). GAPDH served as a loading control.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit antihuman SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, SDS Page, Western Blot, Control

Journal: Scientific reports

Article Title: Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas.

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Figure 5. The effect of SGO2 on cell proliferation and apoptosis (a) The SGO2 knockdown model constructed by siRNA 25 nM transfection into LN229 and GBM8401 cell lines. The knockdown efficiency of SGO2 siRNA or control siRNA in infected LN229 and GBM8401 cells measured by RT-qPCR. Bars, mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.005 showed significant differences. Data are representative of three independent experiments. (b) LN229 and GBM8401 cells were transfected with 25 nM siRNA or siControl. Cell count was determined at the indicated time points. The data are expressed as the mean ± s.d.; n = 3; **p < 0.01, and ***p < 0.001. (c) LN229 and GBM8401 cell with siSGO2 or siControl transfection were labeled with BrdU then proceeded analysis by flow cytometry (**p < 0.01, ***p < 0.005). (d) Cell cycle analysis of LN229 and GBM8401 siSGO2 cells was determined by propidium iodide (PI) stain and flow cytometry. The data are expressed as the mean ± s.d.; n = 3; *p < 0.05, **p < 0.01. (e) Cell apoptosis analysis of LN 229 and GBM8401 siSGO2 cells were determined by tetraethylbenzimidazolylcarbocyanine iodide (JC-1) dye and flow cytometry.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit antihuman SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Knockdown, Construct, Transfection, Control, Infection, Quantitative RT-PCR, Cell Counting, Labeling, Flow Cytometry, Cell Cycle Assay, Staining

Journal: Scientific reports

Article Title: Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas.

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Figure 6. The effect of SGO2 knockdown on cell migration detected by wound-healing assays. Images and Quantitative analysis of LN229 (a) and GBM8401 (b) cells in the wound-healing assay. Data are presented as the mean ± SD (n = 3). *p < 0.05, **p < 0.01.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit antihuman SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Knockdown, Migration, Wound Healing Assay

Journal: Scientific reports

Article Title: Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas.

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Figure 7. The SGO2 protein–protein interaction (PPI) network. (a) In the PPI network established by STRING dataset, SGO2 is a hub protein. (b) The STRING dataset also predicted the association between SGO2, ARUKB, and FOXM1. (c) Protein lysates of LN229 and GBM8401 were applied to SDS-PAGE and Western blot to investigate the protein expression of AURKB and FOXM1(full length blot is presented in Supplementary Fig. 2). α-actinin served as a loading control.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit antihuman SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: SDS Page, Western Blot, Expressing, Control

Journal: Scientific Reports

Article Title: Shugoshin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: The expression level of SGO2 is related to pathological grading of gliomas. The SGO2 mRNA level in different grade of gliomas and non-tumor brain tissue from GEO profile (GDS1962/230165_at/SGO2) ( a ), TCGA ( b ), and CCGA ( c ). SGO2 expression was significantly higher in high-grade gliomas (Grade III and IV) than in low-grade gliomas (Grade II) and non-tumor control. The Y-axis indicates the SGO2 mRNA expression. The p value was adjusted by Bonferroni method in R software (version 3.0.1) between each group.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit anti-human SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Expressing, Control, Software

Journal: Scientific Reports

Article Title: Shugoshin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: The expression level of SGO2 is related to the survival of patients with high-grade gliomas. The Kaplan–Meier survival curve analyzed from GEO profile (GDS1816/230165_at/SGO2) ( a ), TCGA ( b ), and CGGA ( c ). Data showed that patients with high expression of SGO2 had unfavorable survival outcome. (GDS1816/230165_at/SGO2, n = 77, p = 0.0011 by log-rank test, 95% CI 1.000–1.00, hazard ratio 1.001; TCGA, n = 343, p < 1 × 10 –15 by log-rank test, 95% CI 0.87–5.30, hazard ratio 3.90; CGGA, n = 209, p = 1.23 × 10 –11 by log-rank test, 95% CI 2.13–4.25, hazard ratio 3.01).

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit anti-human SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Shugoshin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Validation of SGO2 mRNA and protein levels in glioma cell lines and normal brain tissue ( a ) qRT-PCR was performed to examine SGO2 mRNA expression and the quantitative results are shown in glioma cell lines. The relative expressions were normalized with normal brain. Bars mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.005 showed significant differences. Data are representative of three independent experiments. ( b ) Protein lysates of glioma cell lines, including U87MG, LN229, GBM8401, and U118MG were applied to SDS-PAGE and Western blot analysis to quantitate SGO2 protein expression (full length blot is presented in Supplementary Fig. ). GAPDH served as a loading control.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit anti-human SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, SDS Page, Western Blot, Control

Journal: Scientific Reports

Article Title: Shugoshin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: Validation of SGO2 protein expression in human gliomas and non-tumor brain tissue. Hematoxylin and eosin staining of non-tumor brain tissue ( a ), low grade ( b ) and high grade gliomas ( c ). The immunohistochemical staining of SGO2 on non-tumor brain tissue ( d ), low grade ( e ), and high grade gliomas ( f ) (scale bar: 50 μm). ( g – i ) The SGO2 immunostaining scores in normal brain tissue, low-grade glioma and high-grade glioma were statistically analyzed. The adjusted p value was calibrated between each group.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit anti-human SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Biomarker Discovery, Expressing, Staining, Immunohistochemical staining, Immunostaining

Journal: Scientific Reports

Article Title: Shugoshin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: The effect of SGO2 on cell proliferation and apoptosis ( a ) The SGO2 knockdown model constructed by siRNA 25 nM transfection into LN229 and GBM8401 cell lines. The knockdown efficiency of SGO2 siRNA or control siRNA in infected LN229 and GBM8401 cells measured by RT-qPCR. Bars, mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.005 showed significant differences. Data are representative of three independent experiments. ( b ) LN229 and GBM8401 cells were transfected with 25 nM siRNA or siControl. Cell count was determined at the indicated time points. The data are expressed as the mean ± s.d.; n = 3; ** p < 0.01, and *** p < 0.001. ( c ) LN229 and GBM8401 cell with si SGO2 or siControl transfection were labeled with BrdU then proceeded analysis by flow cytometry (** p < 0.01, *** p < 0.005). ( d ) Cell cycle analysis of LN229 and GBM8401 si SGO2 cells was determined by propidium iodide (PI) stain and flow cytometry. The data are expressed as the mean ± s.d.; n = 3; * p < 0.05, ** p < 0.01. ( e ) Cell apoptosis analysis of LN 229 and GBM8401 si SGO2 cells were determined by tetraethylbenzimidazolylcarbocyanine iodide (JC-1) dye and flow cytometry.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit anti-human SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Knockdown, Construct, Transfection, Control, Infection, Quantitative RT-PCR, Cell Counting, Labeling, Flow Cytometry, Cell Cycle Assay, Staining

Journal: Scientific Reports

Article Title: Shugoshin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: The effect of SGO2 knockdown on cell migration detected by wound-healing assays. Images and Quantitative analysis of LN229 ( a ) and GBM8401 ( b ) cells in the wound-healing assay. Data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit anti-human SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: Knockdown, Migration, Wound Healing Assay

Journal: Scientific Reports

Article Title: Shugoshin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

doi: 10.1038/s41598-021-97119-4

Figure Lengend Snippet: The SGO2 protein–protein interaction (PPI) network. ( a ) In the PPI network established by STRING dataset, SGO2 is a hub protein. ( b ) The STRING dataset also predicted the association between SGO2, ARUKB, and FOXM1. ( c ) Protein lysates of LN229 and GBM8401 were applied to SDS-PAGE and Western blot to investigate the protein expression of AURKB and FOXM1(full length blot is presented in Supplementary Fig. ). α-actinin served as a loading control.

Article Snippet: The tissue microarrays were incubated with a polyclonal rabbit anti-human SGO2 antibody (HPA035163, Atlas Antibodies, Stockholm.

Techniques: SDS Page, Western Blot, Expressing, Control

Journal: eLife

Article Title: Division of labour between PP2A-B56 isoforms at the centromere and kinetochore

doi: 10.7554/elife.42619

Figure Lengend Snippet: Figure 2. Sgo2 specifically localizes B56a to centromeres. (A-G) The effect of Sgo1 and/or Sgo2 knockdown on YFP-B56a localisation in Flp-in HeLa cells. Representative images (A, C, F) and quantifications (B, D, G) of relative kinetochore intensity of B56a in cells arrested in prometaphase with nocodazole after knockdown of Sgo2 (A, B), Sgo1 (C, D), or Sgo1 +Sgo2 (F, G). (E) shows line plots of Sgo2 and B56a localisation following Sgo1 knockdown; 5 kinetochore pairs were analysed per cell, for a total of 10 cells per experiment. Graphs represent the mean intensities (±SD) from 3 independent experiments. Intensity is normalized to the maximum signal present in each channel within the endogenous B56a experiment. (H–M) Flp-in HeLa cells expressing YFP-B56a or YFP-B56g were transfected with the CB-Sgo2 (H–K) or gChr7 +Cas9 DARPIN (L, M) and analysed for B56 recruitment in cells arrested in prometaphase with nocodazole. (H), (L), and J). are representative images; I) and K) are quantifications of relative centromere/kinetochore intensity of the indicated antigen; and M) is quantification of intensity of Sgo2 over B56 at the Chr7 locus. For all centromere/ kinetochore intensity graphs, each dot represents a cell and 10 cells were quantified per experiment for at least 3 independent experiments. The spread of dots indicates the biological variation between individual cells and the errors bars display the variation between the experimental repeats (displayed as -/+SD of the experimental means). Asterisks indicate significance (Mann-Whitney test); ns p>0.05, *p0.05, ****p0.0001. Scale bars, 5mm. DOI: https://doi.org/10.7554/eLife.42619.009

Article Snippet: The following primary antibodies were used for immunofluorescence imaging (at the final concentration indicated): mouse a-GFP (clone 4E12/8, a gift from P. Parker; 1:1000), chicken a-GFP (ab13970, Abcam; 1:5000), mouse a- Sgo1 (clone 3C11, H00151648-M01, Abnova; 1:1000), rabbit a-Sgo2 (A301-262A, Bethyl; 1:1000), mouse a-BubR1 (clone 8G1, 05–898, Upstate/Millipore; 1:1000), mouse a-VSV (clone P5D4, V5507, Sigma; 1:1000), rabbit a-Knl1 (ab70537, Abcam; 1:1000), rabbit a-Bub1 (A300-373A, Bethyl; 1:1000), mouse a-FLAG (clone M2, F3165, Sigma, 1:10000) guinea pig a-Cenp-C (PD030, MBL; 1:5000) and rabbit a-pMELTKnl1 directed against T943 and T1155 of human Knl1 (Nijenhuis et al., 2014), 1:1000).

Techniques: Knockdown, Expressing, Transfection, MANN-WHITNEY

Journal: eLife

Article Title: Division of labour between PP2A-B56 isoforms at the centromere and kinetochore

doi: 10.7554/elife.42619

Figure Lengend Snippet: Figure 6. A C-terminal loop in B56 regulates binding to Sgo2 and LxxIxE motifs to specify centromere/kinetochore localisation. (A-D) Flp-in HeLa cells expressing either YFP-B56a WT or TKHG were transfected with the CB-Sgo2 and analysed for B56 recruitment (A, B) or gChr7 +dCas9 DARPIN to assess YFP-B56a:Sgo2 co-localisation (C, D). Representative images (A, C) and quantification of relative kinetochore intensity (B) or intensity of Sgo2 over B56a at the Chr7 locus (D). For the intensity graphs in B) and D), each dot represents a cell and 10 cells were quantified per experiment from at Figure 6 continued on next page

Article Snippet: The following primary antibodies were used for immunofluorescence imaging (at the final concentration indicated): mouse a-GFP (clone 4E12/8, a gift from P. Parker; 1:1000), chicken a-GFP (ab13970, Abcam; 1:5000), mouse a- Sgo1 (clone 3C11, H00151648-M01, Abnova; 1:1000), rabbit a-Sgo2 (A301-262A, Bethyl; 1:1000), mouse a-BubR1 (clone 8G1, 05–898, Upstate/Millipore; 1:1000), mouse a-VSV (clone P5D4, V5507, Sigma; 1:1000), rabbit a-Knl1 (ab70537, Abcam; 1:1000), rabbit a-Bub1 (A300-373A, Bethyl; 1:1000), mouse a-FLAG (clone M2, F3165, Sigma, 1:10000) guinea pig a-Cenp-C (PD030, MBL; 1:5000) and rabbit a-pMELTKnl1 directed against T943 and T1155 of human Knl1 (Nijenhuis et al., 2014), 1:1000).

Techniques: Binding Assay, Expressing, Transfection